RNA

Part:BBa_K2889001

Designed by: Rui Zhou   Group: iGEM18_Worldshaper-Wuhan   (2018-10-04)


pSB1C3-IL7-AS


IL7-AS can be induced across multiple human and mouse cell types and has been reported to regulate the expression of interleukin-6 (IL6). IL6 is emerging as a major regulator of renal cell cancer.By analyzing data from TANRIC (http://ibl.mdanderson.org/tanric/_design/basic/query.html), IL7-AS was expressed at higher levels in kidney renal clear cell carcinoma (KIRC) than in normal kidney. Next, we examined the expression levels of IL7-AS in 769-P, 786-O and HEK-293T cell lines. IL7-AS expression was markedly higher in 786-O and 769-P cells than in HEK-293T cells. Moreover, overexpression of IL7-AS promoted cell migration of 786-O cells.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2194
    Illegal PstI site found at 167
    Illegal PstI site found at 581
    Illegal PstI site found at 612
    Illegal PstI site found at 735
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1529
    Illegal PstI site found at 167
    Illegal PstI site found at 581
    Illegal PstI site found at 612
    Illegal PstI site found at 735
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2194
    Illegal PstI site found at 167
    Illegal PstI site found at 581
    Illegal PstI site found at 612
    Illegal PstI site found at 735
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2194
    Illegal PstI site found at 167
    Illegal PstI site found at 581
    Illegal PstI site found at 612
    Illegal PstI site found at 735
    Illegal AgeI site found at 1375
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 127
    Illegal BsaI.rc site found at 1183
    Illegal SapI.rc site found at 429


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Categories
Parameters
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